The NNLM All of Us Community Engagement Center (CEC), which coordinates national activities for the NNLM All of Us Community Engagement Network, is located at the University of Iowa Hardin Library for the Health Sciences.

The NNLM All of Us Training and Education Center (TEC) supports the training and education platform for the All of Us Research Program, develops training activities and educational content in conjunction with the NNLM All of Us Community Engagement Center, and provides infrastructure support for the NNLM All of Us National Program. The TEC is located at the University of Pittsburgh Health Sciences Library System.

The NNLM National Center for Data Services (NCDS) coordinates with other NNLM Regions, Offices, and Centers to provide training and resources to increase data science capacity among information professionals, in alignment with the NLM strategic plan.

The NNLM National Evaluation Center (NEC) provides innovative evaluation frameworks and evidence-based evaluation tools and practices to NNLM. The NEC is located at the Galter Health Sciences Library and Learning Center, Northwestern University, Feinberg School of Medicine in Chicago.